This package provides a suite of methods for powerful and robust microbiome data analysis, including data normalization, data simulation, community-level association testing and differential abundance analysis. It implements generalized UniFrac distances, Geometric Mean of Pairwise Ratios (GMPR) normalization, semiparametric data simulator, distance-based statistical methods, and feature- based statistical methods. The distance-based statistical methods include three extensions of PERMANOVA:
PERMANOVA using the Freedman-Lane permutation scheme,
PERMANOVA omnibus test using multiple matrices, and
analytical approach to approximating PERMANOVA p-value.
Feature-based statistical methods include linear model-based methods for differential abundance analysis of zero-inflated high-dimensional compositional data.