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This package provides tools to analyze and visualize Illumina Infinium methylation arrays.
The bayNorm package is used for normalizing single-cell RNA-seq data. The main function is bayNorm, which is a wrapper function for gene specific prior parameter estimation and normalization. The input is a matrix of scRNA-seq data with rows different genes and columns different cells. The output is either point estimates from posterior (2D array) or samples from posterior (3D array).
The data consist of microarrays from 128 different individuals with acute lymphoblastic leukemia (ALL). A number of additional covariates are available. The data have been normalized (using rma) and it is the jointly normalized data that are available here. The data are presented in the form of an exprSet object.
This package provides a number of utility functions for handling single-cell RNA-seq data from droplet technologies such as 10X Genomics. This includes data loading from count matrices or molecule information files, identification of cells from empty droplets, removal of barcode-swapped pseudo-cells, and downsampling of the count matrix.
This package implements a method to rapidly assess cell type identity using both functional and random gene sets and it allows users to quantify cell type replicability across datasets using neighbor voting. MetaNeighbor works on the basis that cells of the same type should have more similar gene expression profiles than cells of different types.
This package provides software and data to support the case studies monograph.
This package provides many functions for computing the nonparametric maximum likelihood estimator (NPMLE) for censored and truncated data.
This package extends the ggplot2 plotting system which implements a grammar of graphics. ggtree is designed for visualization and annotation of phylogenetic trees and other tree-like structures with their annotation data.
The package alpine helps to model bias parameters and then using those parameters to estimate RNA-seq transcript abundance. Alpine is a package for estimating and visualizing many forms of sample-specific biases that can arise in RNA-seq, including fragment length distribution, positional bias on the transcript, read start bias (random hexamer priming), and fragment GC-content (amplification). It also offers bias-corrected estimates of transcript abundance in FPKM(Fragments Per Kilobase of transcript per Million mapped reads). It is currently designed for un-stranded paired-end RNA-seq data.
Rqc is an optimized tool designed for quality control and assessment of high-throughput sequencing data. It performs parallel processing of entire files and produces a report which contains a set of high-resolution graphics.
R-msigdb provides the Molecular Signatures Database in a R accessible objects. Signatures are stored in GeneSet class objects form the GSEABase package and the entire database is stored in a GeneSetCollection object. These data are then hosted on the ExperimentHub. Data used in this package was obtained from the MSigDB of the Broad Institute. Metadata for each gene set is stored along with the gene set in the GeneSet class object.
This package provides classes and methods to support Gene Set Enrichment Analysis (GSEA).
bioassayR is a computational tool that enables simultaneous analysis of thousands of bioassay experiments performed over a diverse set of compounds and biological targets. Unique features include support for large-scale cross-target analyses of both public and custom bioassays, generation of high throughput screening fingerprints (HTSFPs), and an optional preloaded database that provides access to a substantial portion of publicly available bioactivity data.
This package defines data structures for linkage disequilibrium (LD) measures in populations. Its purpose is to simplify handling of existing population-level data for the purpose of flexibly defining LD blocks.
This package provides a data package containing summarized Illumina 450k array data on 2800 samples and summarized EPIC data for 2620 samples. The data can be use as a background data set in the interactive application.
This package is importing data from Illumina SNP experiments and it performs copy number calculations and reports.
This package implements utilities for installation of the basilisk package, primarily for creation of the underlying Conda instance.
This package contains a collection of 9 datasets, andrews and bakulski cord blood, blood gse35069, blood gse35069 chen, blood gse35069 complete, combined cord blood, cord bloo d gse68456, gervin and lyle cord blood, guintivano dlpfc and saliva gse48472. The data are used to estimate cell counts using Extrinsic epigenetic age acceleration (EEAA) method. It also contains a collection of 12 datasets to use with MethylClock package to estimate chronological and gestational DNA methylation with estimators to use with different methylation clocks.
This package computes differentially bound sites from multiple ChIP-seq experiments using affinity (quantitative) data. Also enables occupancy (overlap) analysis and plotting functions.
This package takes sample information in the form of the fraction of mutations in each of 96 trinucleotide contexts and identifies the weighted combination of published signatures that, when summed, most closely reconstructs the mutational profile.
This package provides a database of PROVEAN/SIFT predictions for Homo sapiens dbSNP build 137.
This package provides infrastructure shared by all Biostrings-based genome data packages and support for efficient SNP representation.
This package provides tools to identify cell populations in Flow Cytometry data using non-parametric clustering and segmented-regression-based change point detection.
This package contains the functions to find the gene expression modules that represent the drivers of Kauffman's attractor landscape. The modules are the core attractor pathways that discriminate between different cell types of groups of interest. Each pathway has a set of synexpression groups, which show transcriptionally-coordinated changes in gene expression.